![]() verticillioides, and update the genome annotations based on the improved genome assembly. Here, we aim to produce a gap-free reference genome of F. ![]() verticillioides would enable accurate characterization of the fungal genome function, regulation and evolution, shedding light on mechanisms of growth, development, pathogenicity and mycotoxin production. verticillioides genome assembly available, leaving these essential and complex genomic regions unexplored. Furthermore, no telomere and centromere sequences have been reported in any F. verticillioides has 11 chromosomes suggests the presence of gaps in these assembly versions. Although these genome assemblies have since facilitated the genetic studies of fungal biological processes, they are highly fragmented with several hundreds to thousands of contigs. verticillioides genome assemblies are available in NCBI (National Center for Biotechnology Information) genome database. verticillioides strain 7600 was released in 2010 4 with a contig N50 of 392.3 kb. verticillioides is crucial to both agricultural safety and public health.ĭespite the importance of this fungus, its complete genome sequence has not been assembled and thoroughly analyzed, impeding dissection of molecular and evolutionary mechanisms underlying its pathogenesis, secondary metabolism and drug resistance. Therefore, elucidation of molecular mechanisms underlying fungal pathogenesis and antifungal resistance in F. verticillioides commonly known as Fusariosis has been a surging life threat to the immunocompromised patients due to limited options of antifungal drugs for treatment and emergence of multi-drug resistance 3. veticillioides is considered nonpathogenic to healthy human being, it can become a serious threat to individuals with compromised immune system such as those infected by undergoing organ transplants 2. ![]() verticillioides mycotoxins are fumonisins, a group of polyketide mycotoxins associated with esophageal cancer and neural tube birth defects in human populations consuming the contaminated maize products 1. Besides yield loss, various mycotoxins are produced during fungal infection of maize, reducing the quality of corn products. verticillioides is an excellent resource for further panoramic understanding mechanisms of fungal genome evolution, mycotoxin production and pathogenesis on plant and human host.įusarium verticillioides, a filamentous fungus belonging to Fusarium fujikuroi species complex, causes Fusarium ear rot of maize, a major crop worldwide. In addition, we identified three-dimension chromatin structures such as TADs-like structures and chromatin loops based on Hi-C data of ultra-high coverage. A total of 15,230 protein-coding genes were predicted, 6.2% of which are newly annotated genes. This assembly represents a significant improvement over previous version on contiguity (contig N50: 4.3 Mb), completeness (BUSCO score: 99.0%) and correctness (QV: 88.8). The assembled 42.0 Mb sequence contains eleven gapless chromosomes capturing all centromeres and 19 of all 22 telomeres. verticillioides using PacBio HiFi data and high-throughput chromosome capture (Hi-C) sequencing data. Here, we report a gap-free genome assembly of F. Given its threat to agriculture and public health, genome assembly of this fungus is critical to our understanding of its pathobiology and developing antifungal drugs. Fusarium verticillioides is a filamentous fungus that causes plant diseases and harms human health through cancer-inducing mycotoxin and life-threatening Fusariosis.
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